About the Course
Microorganisms play a major role in the biosphere and within our bodies, but only a tiny fraction has been cultured so far. Microbiome data, that is the genetic information of microorganisms, is therefore an important window into the hidden microbial world.
Microbiome data analysis elucidates the composition of microbial communities and how it changes in response to the environment. When analyzing sequencing data, we learn whether microbial diversity differs across conditions and identify links between microbes. In brief, microbiome data analysis gives us a first idea of how a microbial ecosystem works.
This course will illustrate with the help of real-world example data how to carry out typical analysis tasks, such as comparing microbial composition and diversity, clustering samples and computing associations. If you plan to work with microbiome data, this course will get you up to speed.
What you’ll learn?
- How and why microbiome data are collected
- How to extract species and function counts from sequencing data
- The definitions of richness, evenness and diversity
- How to compare diversity and microbial composition across conditions
- How to compute and interpret taxon and function associations
Module 0: Introduction: Study guide, R basics and a help forum for programming questions
Module 1: Introduction to microbiome data: Sequencing techniques, data types (16S, WGS, metadata), example applications
Module 2: From sequences to counts: Quality control of reads, taxonomic and functional assignment
Module 3: Comparing microbiome: Relative versus absolute abundance, taxonomic and functional richness, evenness and diversity
Module 4: Ordination: Dimension reduction: arranging samples according to their taxonomic and functional composition in two-dimensional space
Module 5: Taxon/function associations: Network construction: computing and interpreting associations between taxa and functions
Module 6: Your favourite microbiome: Databases and journals for microbiome data and guidelines for doing your own analysis
Module 7: Final exam: Complete the course by passing the quiz or by completing a microbiome analysis
- Basic knowledge of biology is required throughout the course.
- Basic knowledge of statistics and programming is required to complete specific sections of this course.
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