Course on Next Generation Sequencing and Metagenomics Analysis at IIT Bombay [Dec 2-6]: Register by Nov 5: Expired

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About the Organiser

The Indian Institute of Technology Bombay (abbreviated as IITB or IIT Bombay) is a public engineering institution located in Powai, Mumbai, India. IIT Bombay was founded in 1958. In 1961, the Parliament decreed IITs as Institutes of National Importance. A committee formed by the Government of India recommended the establishment of four higher institutes of technology to set the direction for the development of technical education in the country in 1946.

Course Overview

The advent of Next Generation Sequencing (NGS) technologies has transformed how biological research is being performed. Today, a human genome can be sequenced in a very short time, giving unprecedented possibilities for investigating human traits, evolution, and diseases. Similarly, whole bacterial communities and their interplay with the environment can be studied, unravelling novel enzymes and organisms. Although less than 1% of most microorganisms can be grown and studied in the lab, with the advent of low-cost high-throughput DNA sequencing technologies, it has become possible to explore the genetic material from entire microbial communities directly without the need of culturing the organisms under study. This emerging field, known as Metagenomics, utilizes a huge genetic reservoir of non-cultivable organisms as a resource for biotechnological and medical products and processes.

As these genomic and metagenomics experiments produce massive amounts of data, bioinformatics skills and supercomputing are crucial for the analysis. The aim of the special course is to give the participant knowledge of the NGS technology with the focus on data analysis. It will qualify the student to understand genomic and metagenomic shotgun sequencing data and enable them to analyze these in a UNIX/Linux working environment.

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Course Objectives

A student who has met the objectives of the course will be able to:

  • Explain the applications of the different NGS technologies, including the weakness and strengths of the approaches.
  • Explain the concept of metagenomics and its applications as opposed to single genomics.
  • Apply for programs in Unix for analysis of NGS data.
  • Execute a proper Quality Assurance of NGS data.
  • Understand and explain the steps involved in a general NGS data analysis.
  • Explain key theoretical concepts of alignment and de novo assembly.
  • Execute gene calling and annotation of a metagenomics sample.
Course Outline
  • Introduction to Bioinformatics and supercomputing
  • History of Sequencing
  • Introduction to UNIX and the shell
  • Hands-on training UNIX and Supercomputing
  • Sequencing technologies
  • Sequencing projects
  • Hands-on training, QC, filtering and trimming (with a break of 20 minutes in-between)
  • Introduction to Metagenomics
  • Human and environmental microbiomes
  • Hands-on training, mapping and de-novo assembly
  • Gene prediction, genome annotation
  • Guidelines to Statistical Analysis of Microbial Composition Data Inferred from Metagenomic Sequencing
  • Hands-on training, gene calling and annotation
  • Restructuring metagenomics data
  • Abundance binning
  • Hands-on training, metagenomic species identification
  • Summary of the course
Who may Benefit?
  • You are an executive or an engineer or a researcher from a manufacturing, service industry or a government organization/Institution including R&D laboratories
  • You are a faculty from an academic institution interested in learning about genomics and metagenomics.
  • You are a student at all levels (BTech/MSc/MTech/PhD) aspiring to learn about genomics and metagenomics.
  • You are keen on upgrading your bioinformatics skills.

The participation fee (including taxes) for taking the course for different categories is as follows:

  • Academia (College teachers only): INR 5900
  • Govt. Organisation: INR 8850
  • Industry: INR 23600
  • Overseas / Foreign National: INR 29500
  • Student (Full Time): INR 1770
How to Register?

Interested candidate can register for the course through this link.

All completed registration forms (available here) with bank transaction details may be mailed to kirankondabagil[at]


IIT, Bombay [Guest House- Jalvihar Conference Hall]


Prof. Kiran Kondabagil
Phone:  +91-22-25767758
E-mail: kirankondabagil[at]

For more details, click here.

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